Don't know which ship but official sea trials were first conducted in 2002. BTW - the aircraft broke during these trials!
If you are a carrier aviator, it’s “the boat” not “ship”
Official Sea Trials on a CV/CVN, aka boat, have not been executed for the V-22 prior to now. Sea Trials involves a syllabus of KPPs; Key Performance Parameters. The first boat to conduct Sea Trials for the Osprey was the Wasp in December of 1990. The second boat to conduct Sea Trials for the Osprey was the Saipan in January of 1999. Wasp is a LHD. Saipan is a LHA. Just landing on the deck of a CV/CVN doesn’t constitute executing Sea Trials.