The horses and lizards and turtles and scorpions and tarantulas and rabbits and rattlers all know the freeway is there; they aren’t stupid. And since the highway is existing already adding two more lanes isn’t going to impact much, except maybe improve the air quality because there will be less congestion and so less accidents. And more happy feelings radiating out into the universe from happy drivers. And feelings of happiness filling the universe is what counts.
I sincerely doubt that the tarantula population is cognizant of the Interstate.