Posted on 10/16/2014 12:09:10 AM PDT by Chgogal
RISK GROUP CLASSIFICATION: Risk Group 4 (38).
CONTAINMENT REQUIREMENTS: Containment Level 4 facilities, equipment, and operational practices for work involving infectious or potentially infectious materials, animals, and cultures.
PROTECTIVE CLOTHING: Personnel entering the laboratory must remove street clothing, including undergarments, and jewellery, and change into dedicated laboratory clothing and shoes, or don full coverage protective clothing (i.e., completely covering all street clothing). Additional protection may be worn over laboratory clothing when infectious materials are directly handled, such as solid-front gowns with tight fitting wrists, gloves, and respiratory protection. Eye protection must be used where there is a known or potential risk of exposure to splashes (39).
OTHER PRECAUTIONS: All activities with infectious material should be conducted in a biological safety cabinet (BSC) in combination with a positive pressure suit, or within a class III BSC line. Centrifugation of infected materials must be carried out in closed containers placed in sealed safety cups, or in rotors that are unloaded in a biological safety cabinet. The integrity of positive pressure suits must be routinely checked for leaks. The use of needles, syringes, and other sharp objects should be strictly limited. Open wounds, cuts, scratches, and grazes should be covered with waterproof dressings. Additional precautions should be considered with work involving animal activities (39).
SUSCEPTIBILITY TO DISINFECTANTS: Ebola virus is susceptible to sodium hypochlorite, lipid solvents, phenolic disinfectants, peracetic acid, methyl alcohol, ether, sodium deoxycholate, 2% glutaraldehyde, 0.25% Triton X-100, β-propiolactone, 3% acetic acid (pH 2.5), formaldehyde and paraformaldehyde, and detergents such as SDS (20, 21, 31-34).
PHYSICAL INACTIVATION: Ebola are moderately thermolabile and can be inactivated by heating for 30 minutes to 60 minutes at 60ºC, boiling for 5 minutes, gamma irradiation (1.2 x106 rads to 1.27 x106 rads), and/or UV radiation (3, 6, 20, 32, 33).
SURVIVAL OUTSIDE HOST: The virus can survive in liquid or dried material for a number of days (23). Infectivity is found to be stable at room temperature or at 4°C for several days, and indefinitely stable at -70°C (6, 20). Infectivity can be preserved by lyophilisation.
Thanks for the info. Whenever I see a MSDS it is for a chemical. As in if you have it at your business, shipping it, etc.
“I’m sorry sir, you have Ebola but not an MSDS? That is an OSHA violation and I’ll have to write you up.”
Bottom line - Ebola is categorized as Risk Group 4 which means the US can NOT handle a “wide spread” epidemic. Period.
“..and respiratory protection...”
Kind of open-ended on that one. Real MSDS sheets have specific requirements of what types of filters, etc. are needed.
SURVIVAL OUTSIDE HOST: The virus can survive in liquid or dried material for a number of days (23). Infectivity is found to be stable at room temperature or at 4°C for several days, and indefinitely stable at -70°C (6, 20). Infectivity can be preserved by lyophilisation.
Baghdad Obola would deny it’s a risk 4.
I’ll stick with this as I am simple-minded as well.
I’m pretty sure the “...number of days (23)”; the (23) refers to a cited reference, and not the number of days it can survive outside the host. In some cases (dry??) it can survive many hours. In other cases (moist, wet?) in can last up to 6 days.
I wasn’t precise. The temperatures impressed me.
“The temperatures impressed me.”
I shop at the thrift stores and put stuff in a bag and then in my freezer for a couple of days (kills lice, bedbugs, etc.)
Pretty sure mine doesn’t get down to -70 C!
Says susceptible to sodium hypochlorite. Wonder if calcium hypochlorite is good too? Lot of us have it for water disinfection. Also wonder about household bleach. The sodium is a lot harder to get and use I think.
Household bleach (Wal-Mart Great Value brand) is sodium hypochlorite 8.25%.
Bleach will become the scent of the future.
Worth repeating:
“SURVIVAL OUTSIDE HOST: The virus can survive in liquid or dried material for a number of days (23).”
Wait, what? Whither “bodily fluids only”?
This is a family website. Watch your language.
Thank you for posting.
“The virus can survive in liquid or dried material for a number of days (23).”
Or must be boiled for 5 minutes to kill virus.
uhm...I HOPE 23 is a cited footnote
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Household bleach (Wal-Mart Great Value brand) is sodium hypochlorite 8.25%.
Common white vinegar = acetic acid 3%, pH 2.5 available cheap in gallon jugs
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