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Evolution Caught In The Act
ScienceDaily ^ | 20 February 2004 | Staff

Posted on 02/20/2004 10:58:13 AM PST by PatrickHenry

ANN ARBOR, Mich. -- An experiment which forced E. coli bacteria to adapt or perish showed that, in a pinch, they were capable of improvising a novel molecular tool to save their skins.

"The bacteria reached for a tool that they had, and made it do something it doesn't normally do," said James Bardwell, an associate professor of molecular, cellular and developmental biology at the University of Michigan. "We caught evolution in the act of making a big step."

This big step also turns out to be a new way of making molecular bolts called disulfide bonds, which are of particular interest to the biotechnology industry. Disulfide bonds are stiffening struts in proteins that also help the proteins fold into their proper, functional, three-dimensional shapes.

In a paper published Feb. 20 in the journal Science, a joint research team at the University of Michigan and the University of Texas describes how a resourceful bacterium was able to develop an entirely new way to make disulfide bonds. This restarted its motor and enabled it to move toward food before it starved to death.

The laboratory of George Georgiou, a professor of chemical and biomedical engineering at UT Austin, used a strain of mutant bacteria developed by Bardwell that had lost their ability to make disulfide bonds. These disulfides are critical for the ability of the bacteria's propeller-like swimming motor, the flagellum, to work. The researchers then put these non-swimming bugs to the test by placing them on a dish of food where, once they had exhausted the food they could reach, they either had to repair that broken motor or starve to death on the spot.

The bacteria used in the experiment were forced to use a protein called thioredoxin---which normally destroys disulfide bonds---to make the bonds instead. In a process similar to natural selection, UT graduate student Lluis Masip made random alterations in the DNA encoding thioredoxin and then subjected thousands of bacteria to the swim-or-starve test. He wanted to see if an altered version of thioredoxin could be coerced to make disulfides for other proteins in the bacteria.

To the researchers' surprise, a mutant carrying only two amino acid changes, amounting to less than 2 percent of the total number of amino acids in thioredoxin, restored the ability of the bacteria to move. The altered thioredoxin was able to carry out disulfide bond formation in numerous other bacterial proteins all by itself, without relying on any of the components of the natural disulfide bond pathway. The mutant bug managed to solve the problem in time and swim away from starvation and multiply.

When Bardwell heard Georgiou discuss the experiment at a scientific meeting in France in spring 2002, he asked if they could collaborate on figuring out what, precisely, the successful bacterium had managed to accomplish.

With post-doctoral fellow Jean Francois Collet, Bardwell's team found that the two amino acid substitutions in thioredoxin cause a remarkable transformation: they result in the binding of two iron and two sulfur atoms that form a complex that bridges two thioredoxin protein molecules. This iron-sulfur cluster was shown to be necessary for the new enzyme to form disulfides. Iron-sulfur complexes occur in many enzymes, but never before had such a functional iron-sulfur complex been introduced into a protein as a result of laboratory evolution. James Penner-Hahn, a professor of chemistry and chair of the Biophysics Research Division at U-M, showed exactly what kind of iron-sulfur cluster was involved.

Bardwell likens the new pathway for disulfide bond formation to engineering. "People often speak of Computer Assisted Design (CAD), where you try things out on a computer screen before you manufacture them. We put the bacteria we were working on under a strong genetic selection, like what can happen in evolution, and the bacteria came up with a completely new answer to the problem of how to form disulfide bonds. I think we can now talk about Genetic Assisted Design (GAD).

"The naturally occurring enzymes involved in disulfide bond formation are a biological pathway whose main features are the same from bacteria to man," Bardwell said. "Understanding how disulfide bond formation occurs and figuring out new ways to make it happen could be important to numerous disease states, like Alzheimer's and cystic fibrosis, that result from defective protein folding. "

Disulfides are also vital for the activity of most proteins that are injected into people for medical purposes, such as insulin and TPA, a blood clot dissolver that is injected into people who are having heart attacks and strokes.

This story has been adapted from a news release issued by University Of Michigan.


TOPICS: Culture/Society; Miscellaneous; Philosophy
KEYWORDS: creationism; crevolist; darwin; evolution
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Bold font added by your humble poster. Everyone be nice.
1 posted on 02/20/2004 10:58:13 AM PST by PatrickHenry
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To: *crevo_list; VadeRetro; jennyp; Junior; longshadow; RadioAstronomer; Physicist; LogicWings; ...
PING. [This ping list is for the evolution side of evolution threads, and sometimes for other science topics. FReepmail me to be added or dropped.]
2 posted on 02/20/2004 10:59:07 AM PST by PatrickHenry (The universe is made for life, therefore ID. Life can't arise naturally, therefore ID.)
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To: PatrickHenry
Thanks for the ping!
3 posted on 02/20/2004 11:01:06 AM PST by Alamo-Girl
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To: PatrickHenry
This proves ID! Quick! Remove it!

Seriously, great article.

4 posted on 02/20/2004 11:02:25 AM PST by Shryke
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To: PatrickHenry
I always carry a paddle just in case my motor stops.

CG
5 posted on 02/20/2004 11:02:38 AM PST by Conspiracy Guy (I'm not stupid. I just act that way.)
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To: Ahban
Implications here for your arguments on another thread.

(Please don't anyone bother to tell me they're still bacteria.)
6 posted on 02/20/2004 11:02:40 AM PST by VadeRetro
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To: PatrickHenry
Could it have been happened without altering the DNA ?
evolution caught in the act ? nice try
7 posted on 02/20/2004 11:04:16 AM PST by stylin19a (Is it vietnam yet ?)
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To: VadeRetro
One small step for bacteria................
8 posted on 02/20/2004 11:05:56 AM PST by Seeking the truth (Some oldies/newbies are really full of themselves, aren't they?)
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To: PatrickHenry
Yes. Silliness such as this is what evos always give us to "prove" evolution. No fossil record. No transitional species. Just microbes that change a bit due to stimuli. I'm so convinced.
9 posted on 02/20/2004 11:06:36 AM PST by Timmy
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To: PatrickHenry
"The bacteria reached for a tool that they had, and made it do something it doesn't normally do,"

and then:

"disulfide bond formation are a biological pathway whose main features are the same from bacteria to man"

That's not evolution, that's just learning more about what bacteria can or cannot do. It's a quality which it already possessed of which James Bardwel and friends, were previously unaware. The only 'evolution' here is in the scientist understanding of bacteria.

Disulfide bond formation is a seemingly common operation already in use by a multiple of living creatures. We just didnt know about it it. They did.

10 posted on 02/20/2004 11:09:50 AM PST by antonia
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To: PatrickHenry
This is VERY interesting, evidence of evolution or not.
11 posted on 02/20/2004 11:09:54 AM PST by Paradox (Cogito ergo moon.)
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To: Timmy
No fossil record. No transitional species.

You've never been on a thread where we mentioned any of that stuff? Really?

Here, let me redress your incredible bad luck.

12 posted on 02/20/2004 11:09:59 AM PST by VadeRetro
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To: PatrickHenry
[ The mutant bug managed to solve the problem in time and swim away from starvation and multiply. ]

Lol... reminds me of when lizards decided to grow feathers... and after that.. learn to FLY...

13 posted on 02/20/2004 11:11:39 AM PST by hosepipe
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To: antonia
That's not evolution, that's just learning more about what bacteria can or cannot do. It's a quality which it already possessed of which James Bardwel and friends, were previously unaware. The only 'evolution' here is in the scientist understanding of bacteria.

They couldn't all do it. Only the carriers of an identifiable and identified mutation survived. Try again.

14 posted on 02/20/2004 11:11:55 AM PST by VadeRetro
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To: antonia
That's not evolution, that's just learning more about what bacteria can or cannot do. It's a quality which it already possessed of which James Bardwel and friends, were previously unaware. The only 'evolution' here is in the scientist understanding of bacteria.

Disulfide bond formation is a seemingly common operation already in use by a multiple of living creatures. We just didnt know about it it. They did.

I think you've misunderstood the article. Perhaps you should re-read it.

15 posted on 02/20/2004 11:12:13 AM PST by general_re (Ubi solitudinem faciunt, pacem appellant. - Tacitus)
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To: PatrickHenry
We're seeing an awful lot of really panicky instant denial on this thread.
16 posted on 02/20/2004 11:13:13 AM PST by VadeRetro
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To: stylin19a
Could it have been happened without altering the DNA ?

Humans did not design the DNA difference. Random variation and natural selection.

17 posted on 02/20/2004 11:14:03 AM PST by VadeRetro
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To: All
Sounds to me as if they manipulated the bacteria on a genetic level to achieve just this result. If anything, this proves INTELLEGENT DESIGN more than it does evolution.
18 posted on 02/20/2004 11:14:03 AM PST by Wombat101 (Sanitized for YOUR protection....)
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To: hosepipe
Lol... reminds me of when lizards decided to grow feathers... and after that.. learn to FLY...

I'm not aware of anyone claiming that lizards "decided" to grow feathers or learn to fly. Perhaps you could point to an example of someone saying that?

Surely you're not just making up bogus claims to discredit your opponents for statements that they've never made, right?
19 posted on 02/20/2004 11:15:17 AM PST by Dimensio (I gave you LIFE! I -- AAAAAAAAH!)
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To: Wombat101
Sounds to me as if they manipulated the bacteria on a genetic level to achieve just this result.

Read it again. They reverse-engineered the successful bacteria strain after it emerged from the test. You don't have to reverse-engineer your own design.

20 posted on 02/20/2004 11:15:30 AM PST by VadeRetro
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